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Essai de cryoconservation des gamétophytes de l'algue alimentaire Undaria pinnatifida (Laminariale)
Provedor de dados: 5
Autores: Arbault, Suzanne; Renard, Philippe; Pérez, Rene; Kaas, Raymond.
Low temperature tolerance was determined for gametophytes of food alga Undaria pinnatifida according to various cryopreservation methods. Survival durations after storage in a medium containing 28% glycerol at −80 °C or in a 5-10% glycerol solution at −196 °C were only 9 and 4 days respectively. On the other hand, cooling at 5 °C/min. and storage at −15 °C for 15 minutes, without cryoprotective medium, did not reduce viability but this was highly decreased by storage for 15 minutes at −30 or −196 °C, with or without DMSO. The morphological cooling injuries were depigmentation and/or a contraction of the cells.
Tipo: Text
Palavras-chave: Algue; Cryoconservation; Gamétophyte; Undaria pinnatifida; Alga; Cryopreservation; Gametophyte; Undaria pinnatifida.
Ano: 1990
URL: http://archimer.ifremer.fr/doc/00189/30071/28559.pdf